The PacBio RS II uses Single Molecule, Real-Time (SMRT®) technology for generating long reads. It is ideal for whole genome sequencing of small genomes and targeted sequencing.
Single Molecule, Real-Time (SMRT®) technology is built upon two key innovations that overcome major challenges in the field of sequencing. ZeroMode Waveguides (ZMWs) allow light to illuminate only the bottom of a well in which a DNA polymerase/template complex is immobilized. Phospholinked nucleotides allow observation of the immobilized complex as the DNA polymerase produces a completely natural DNA strand. The key features are:
- Long Read Lengths
- High Consensus Accuracy
- Low Degree of Bias
- Simultaneous Epigenetic Characterization