- Illumina TruSeq Stranded. Whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
|Input material||Total RNA: 2 µg mRNA: small RNA/ MiRNA: 1,5 µg||Total RNA: 200 ng mRNA: 20 ng||Total RNA: 4 µg mRNA: 800 ng|
|Sample volume||Total RNA: 20 µL mRNA: 20 µL sRNA/ MiRNA: 5 µL||Total RNA: 10 µL mRNA: 10 µL sRNA: 10 µL||Total RNA: 50 µL mRNA: 20 µL|
|OD 260/230 OD 260/280 RIN score||+/- 2 +/- 2 > 8||1,5: Additional purification (extra cost) 1,5: Additional purification (extra cost)|
- Lexogen QuantSeq (Gene expression analysis only). The QuantSeq FWD Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
|Input material||1 µg||100 ng|
|Sample volume||10 µL||5 µL|
All samples should be stored and shipped at -80 °C. In case your samples don’t meet the requirements, please contact us at firstname.lastname@example.org.
For all projects, please make sure to always schedule a meeting with us before bringing your samples.
To know more about RNA-Seq at Genomics Core Leuven, please visit our RNA-Seq protocols and pipelines section.