RNA sequencing (RNA-seq)
Input requirements:
Sample Requirements
- Illumina TruSeq Stranded. Whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
|
Criteria |
Optimal |
Minimum |
Maximum |
Input material
|
Total RNA: 2 µg mRNA: small RNA/ MiRNA: 1,5 µg |
Total RNA: 200 ng mRNA: 20 ng
|
Total RNA: 4 µg mRNA: 800 ng
|
Sample volume
|
Total RNA: 20 µL mRNA: 20 µL sRNA/ MiRNA: 5 µL |
Total RNA: 10 µL
mRNA: 10 µL
sRNA: 10 µL
|
Total RNA: 50 µL
mRNA: 20 µL
|
OD 260/230 OD 260/280 RIN score |
+/- 2 +/- 2 > 8 |
1,5: Additional purification (extra cost) 1,5: Additional purification (extra cost) |
- Lexogen QuantSeq (Gene expression analysis only). The QuantSeq FWD Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
|
Criteria |
Optimal |
Minimum |
Input material
|
1 µg
|
100 ng
|
Sample volume
|
10 µL
|
5 µL
|
All samples should be stored and shipped at -80 °C. In case your samples don’t meet the requirements, please contact us at rnaseq@genomicscore.be.
For all projects, please make sure to always schedule a meeting with us before bringing your samples.
To know more about RNA-Seq at Genomics Core Leuven, please visit our RNA-Seq protocols and pipelines section.
Publications we have been involved