RNA sequencing (RNA-seq)

Input requirements:

  • RNA samples with GC code labels
  • Pairwise groups (GC codes) to compare for DE Analysis


  • Raw data: fastq-files
  • Aligned data: BAM-file
  • Counting data data: txt file
  • Differential expression report

How to request this service:

Sample Requirements

  • Illumina TruSeq Stranded. Whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.





Input material
Total RNA: 2 µg


small RNA/ MiRNA: 1,5 µg
Total RNA: 200 ng

mRNA: 20 ng


Total RNA: 4 µg

mRNA: 800 ng


Sample volume
Total RNA: 20 µL

mRNA: 20 µL

sRNA/ MiRNA: 5 µL
Total RNA: 10 µL

mRNA: 10 µL

sRNA:  10 µL
Total RNA: 50 µL

mRNA: 20 µL
OD 260/230

OD 260/280

RIN score
+/- 2

+/- 2

> 8
1,5: Additional purification (extra cost)

1,5: Additional purification (extra cost)
  • Lexogen QuantSeq (Gene expression analysis only). The QuantSeq FWD Kit is a library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.




Input material
1 µg
100 ng
Sample volume
10 µL
 5 µL

All samples should be stored and shipped at -80 °C. In case your samples don’t meet the requirements, please contact us at rnaseq@genomicscore.be.

For all projects, please make sure to always schedule a meeting with us before bringing your samples.

To know more about RNA-Seq at Genomics Core Leuven, please visit our RNA-Seq protocols and pipelines section.

Publications we have been involved